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Atomic Force Microscopy (AFM)
- STRUCTURAL BIOLOGY
Contact: Dr. Volker Walhorn |
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Research Topic: Structural information of individual functional
molecules and complexes can be investigated by in-situ atomic
force microscopy (AFM) by immobilising the biomolecules of interest
(nucleic acids, proteins, carbohydrates, …) via physical,
chemical or biological interaction on a flat surface or directly
embedded in the cellular membrane environment. The interesting
structural information includes sub-nm-conformation, molecular
symmetry, binding location and molecular temporal dynamics.
The following typical examples are typical results of actual
research projects where biological processes of higher complexity
(transcription regulation,molecular motors, self-assembly of
2D-protein s-layers) are investigated.
We currently run several commercial AFM for experiments under ambient and liquid conditions, self-built combined TIRF/AFM and SNOM microscopes, as very recently a novel STM/AFM-microscope for ultra-high-vacuum applications. |
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Images: DNA-protein complex (left), V-ATPase
(second left, middle), bacterial S-layer (second right, right). |
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References: ... see section Publications |
Last updated: 06.02.2017
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