Atomic Force Microscopy (AFM) - STRUCTURAL BIOLOGY

Contact: Dr. Volker Walhorn

    Research Topic: Structural information of individual functional molecules and complexes can be investigated by in-situ atomic force microscopy (AFM) by immobilising the biomolecules of interest (nucleic acids, proteins, carbohydrates, …) via physical, chemical or biological interaction on a flat surface or directly embedded in the cellular membrane environment. The interesting structural information includes sub-nm-conformation, molecular symmetry, binding location and molecular temporal dynamics. The following typical examples are typical results of actual research projects where biological processes of higher complexity (transcription regulation,molecular motors, self-assembly of 2D-protein s-layers) are investigated.
We currently run several commercial AFM for experiments under ambient and liquid conditions, self-built combined TIRF/AFM and SNOM microscopes, as very recently a novel STM/AFM-microscope for ultra-high-vacuum applications.
    Images: DNA-protein complex (left), V-ATPase (second left, middle), bacterial S-layer (second right, right).
    References: ... see section Publications

Last updated: 06.02.2017