Lecture Advanced Light Microscopy

There is an updated version of this lecture, the slides can be found here

Lecture 1: Microscopy, Magnification, Point-spread function
Lecture 2: Background and Noise, Light sources and Illumination
Lecture 3: Basic fluorescent microscope setup, TIRF
Lecture 4: TIRF (short lecture)
Lecture 5: Introduction to fluorescent dyes
Lecture 6: Introduction to cells (not online here due to copyright)
Lecture 7: Laser scanning microscope
Lecture 8: No lecture slides: Hands-on laser scanning microscope
Lecture 9: Image capture: History, CCD and CMOS cameras
Lecture 10: Digital images: ADCs, bit depth, image processing
Lecture 11: Structured illumination: General concept, multi-spot confocal, background estimation
Lecture 12: Structured illumination: Lateral superresolution through structured illumination

All slides, sketches and figures can be re-used under CC-BY-SA-4.0 license.

last update: 2015-03-15