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Toxicity of Cell-Nanoparticle-Interaction
- NANOCARE / NANOTOXICITY
Contact: Dr. Katja Tönsing |
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The binding and uptake of nanoparticles (NP)
by living cells is of central interest for an investigation
of possible interference and modulations of cellular processes.
These dynamic procceses have to be quantified and related to
cell physiological and toxicological results. We use atomic
force microscopy, AFM force spectroscopy and 2-photon-laser
scanning micropscopy in order to investigate binding location,
nanoparticle translocation and interaction at the level of individual
nanoparticles. |
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Images: (a) AFM image of living rat lung cell,
(b) AFM image of nanoparticles at cell membrane (tip image with
from small NP), and c) 2PLSM image of NP endocytosis in living
cell. |
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Project 1 |
Nanoparticle-Cell Interaction |
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The binding and uptake of distinct nanoparticle
systems in rat lung epthelial cells (RLE-6TN) is currently investigated
and quantified with AFM and 2-photon-laser scanning micropscopy.
In general we aim for a correlation of biophysical cell parameters
with dynamic nanoparticle endo/exocytosis and related physiological/toxicological
data. |
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Publications: [see section "Publications"] |
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Collaboration: J. Schneckenburger, University
of Münster |
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other ongoing projects [...] |
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We acknowledge funding from BMBF within
the project NANOCARE. |
