| |
 |
Nanopores - SINGLE MOLECULE
TRANSLOCATION
Contact: Dr. Andy Sischka |
| |
|
|
| |
|
The translocation dynamics of individual macromolecules
like proteins (EcoRI, RecA, peroxiredoxines) is quantitativly
investigated through solid-state nanopores. Beyond looking into
the physical mechanisms involved we explore the possibility
of future sequencing and diagnostic applications thereof. |
| |
|
|
| |
|
 |
| |
|
Images: Solid state nanopor with a diameter
of 30 nm (left). Experimental concept with OTmanipulation (middle).
Experimental results from single protein (EcoRI) translocation
(right). |
| |
|
|
| |
Project 1 |
Manipulation and Threading of Single DNA
Molecules through Nanopores |
| |
|
Individual DNA molecules are manipulated and
navigated in the vicinity of a nanopore. For that, we introduced
a versatile and high precision three-dimensional optical tweezers
setup with minimal optical interference to measure the small
and minute forces and manipulate single molecules in the vicinity
of a weak reflective surface. Our tweezers system integrates
an inverted optical microscope with a single IR-laser beam that
is spatially filtered in an appropriate way to allow force measurements
in three dimensions with remarkably high precision when operated
in backscattered light detection mode.
The setup was tested by overstretching Lambda-DNA in x and z
directions perpendicular and along the optical axis, and by
manipulating individual Lambda-DNA molecules in the vicinity
of a nanopore thatallowed quantitative single molecule threading
experiments with minimal optical interference |
| |
|
|
| |
|
Publications: [see section "Publications"] |
| |
|
Collaborations: P. Reimann, Soft Matter Theory;
N. Sewald, Dept. of Bioorganic Chemistry, Bielefeld University;
and T. Glaser, Dept. of Anorganic Chemistry, Bielefeld University |
| |
|
|
| |
|
other ongoing projects [...] |
| |
|
|
| |
|
We acknowledge funding from DFG within
SFB 613 (Germany) |
